Summary
A method is described for the isolation of milligram quantities of viable, hormone-responsive xanthophores from goldfish scales. The preparations are typically 70 to 90% pure and are useful for biochemical analyses or for establishing primary cultures.
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References
Bagnara, J. T.; Hadley, M. E. Chromatophores and color change. Englewood Cliffs, NJ: Prentice Hall; 1973.
Winchester, J. D.; Ngo, F.; Tchen, T. T.; Taylor, J. D. Hormone-induced dispersion or aggregation of carotenoid-containing smooth endoplasmic reticulum in cultured xanthophores from the goldfish,Carassius auratus L. Endocr. Res. Commun. 3: 335–342; 1976.
Obika, M.; Lo, S. J.; Tchen, T. T.; Taylor, J. D. Ultrastructural demonstration of hormone-induced movement of carotenoid droplets and endoplasmic reticulum in xanthophores of the goldfish,Carassius auratus L. Cell Tiss. Res. 190: 409–416; 1978.
Lynch, T. J.; Lo, S. J.; Taylor, J. D.; Tchen, T. T. Characterization of and hormonal effects on subcellular fractions from xanthophores of the goldfishCarassius auratus L. Biochem. Biophys. Res. Commun. 102: 127–134; 1981.
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Preliminary results of this study were reported at the 11th International Pigment Cell Conference.
This research was supported by Grant AM-13724 from the U.S. Public Health Service.
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Lo, S.J., Grabowski, S.M., Lynch, T.J. et al. Isolation of xanthophores from the goldfish (Carassius auratus L.). In Vitro 18, 356–360 (1982). https://doi.org/10.1007/BF02796335
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DOI: https://doi.org/10.1007/BF02796335

