PIAS4 explained

E3 SUMO-protein ligase PIAS4 is one of several protein inhibitor of activated STAT (PIAS) proteins. It is also known as a protein inhibitor of activated STAT protein gamma (PIASg or PIASy), and is an enzyme that in humans is encoded by the PIAS4 gene.^{[1] [2]}

Interactions

PIAS4 has been shown to interact with Mothers against decapentaplegic homolog 6,^[3] Mothers against decapentaplegic homolog 7^[3] and Lymphoid enhancer-binding factor 1.^[4] PIAS4 has been shown to be responsible for the SUMOylation of inhibited Werner syndrome helicase (WRN) trapped on DNA in microsatellite unstable cancer cells. ^[5]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Liu B, Gross M, ten Hoeve J, Shuai K . A transcriptional corepressor of Stat1 with an essential LXXLL signature motif . Proc. Natl. Acad. Sci. U.S.A. . 98 . 6 . 3203–7 . 2001 . 11248056 . 10.1073/pnas.051489598 . 30631 . 2001PNAS...98.3203L . free .
• Nelson V, Davis GE, Maxwell SA . A putative protein inhibitor of activated STAT (PIASy) interacts with p53 and inhibits p53-mediated transactivation but not apoptosis . Apoptosis . 6 . 3 . 221–34 . 2001 . 11388671 . 10.1023/A:1011392811628 . 13435653 .
• Gross M, Liu B, Tan J, etal . Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells . Oncogene . 20 . 29 . 3880–7 . 2001 . 11439351 . 10.1038/sj.onc.1204489 . free .
• Sachdev S, Bruhn L, Sieber H, etal . PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies . Genes Dev. . 15 . 23 . 3088–103 . 2001 . 11731474 . 10.1101/gad.944801 . 312834 .
• Ohmine K, Ota J, Ueda M, etal . Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells . Oncogene . 20 . 57 . 8249–57 . 2002 . 11781839 . 10.1038/sj.onc.1205029 . free .
• Tan JA, Hall SH, Hamil KG, etal . Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators . J. Biol. Chem. . 277 . 19 . 16993–7001 . 2002 . 11877418 . 10.1074/jbc.M109217200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Subramanian L, Benson MD, Iñiguez-Lluhí JA . A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3 . J. Biol. Chem. . 278 . 11 . 9134–41 . 2003 . 12511558 . 10.1074/jbc.M210440200 . free .
• Dahle Ø, Andersen TØ, Nordgård O, etal . Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner . Eur. J. Biochem. . 270 . 6 . 1338–48 . 2003 . 12631292 . 10.1046/j.1432-1033.2003.03504.x . free .
• Yamamoto H, Ihara M, Matsuura Y, Kikuchi A . Sumoylation is involved in beta-catenin-dependent activation of Tcf-4 . EMBO J. . 22 . 9 . 2047–59 . 2003 . 12727872 . 10.1093/emboj/cdg204 . 156076 .
• Lee PS, Chang C, Liu D, Derynck R . Sumoylation of Smad4, the common Smad mediator of transforming growth factor-beta family signaling . J. Biol. Chem. . 278 . 30 . 27853–63 . 2003 . 12740389 . 10.1074/jbc.M301755200 . free .
• Chun TH, Itoh H, Subramanian L, etal . Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy . Circ. Res. . 92 . 11 . 1201–8 . 2003 . 12750312 . 10.1161/01.RES.0000076893.70898.36 . free .
• Imoto S, Sugiyama K, Muromoto R, etal . Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3 . J. Biol. Chem. . 278 . 36 . 34253–8 . 2003 . 12815042 . 10.1074/jbc.M304961200 . free . 2115/28123 . free .
• Galleguillos D, Vecchiola A, Fuentealba JA, etal . PIASgamma represses the transcriptional activation induced by the nuclear receptor Nurr1 . J. Biol. Chem. . 279 . 3 . 2005–11 . 2004 . 14559918 . 10.1074/jbc.M308113200 . free . 10533/175295 . free .
• Gross M, Yang R, Top I, etal . PIASy-mediated repression of the androgen receptor is independent of sumoylation . Oncogene . 23 . 17 . 3059–66 . 2004 . 14981544 . 10.1038/sj.onc.1207443 . free .
• Zoumpoulidou G, Jones MC, Fernandez de Mattos S, etal . Convergence of interferon-gamma and progesterone signaling pathways in human endometrium: role of PIASy (protein inhibitor of activated signal transducer and activator of transcription-y) . Mol. Endocrinol. . 18 . 8 . 1988–99 . 2005 . 15155784 . 10.1210/me.2003-0467 . free .
• Imoto S, Sugiyama K, Yamamoto T, Matsuda T . The RING domain of PIASy is involved in the suppression of bone morphogenetic protein-signaling pathway . Biochem. Biophys. Res. Commun. . 319 . 1 . 275–82 . 2004 . 15158472 . 10.1016/j.bbrc.2004.04.161 . 2004BBRC..319..275I . 2115/28116 . free .

Notes and References

1. Liu B, Liao J, Rao X, Kushner SA, Chung CD, Chang DD, Shuai K . Inhibition of Stat1-mediated gene activation by PIAS1 . Proc Natl Acad Sci U S A . 95 . 18 . 10626–31 . Sep 1998 . 9724754 . 27945 . 10.1073/pnas.95.18.10626 . 1998PNAS...9510626L . free .
2. Web site: Entrez Gene: PIAS4 Protein inhibitor of activated STAT, 4.
3. Imoto . Seiyu . Sugiyama Kenji . Muromoto Ryuta . Sato Noriko . Yamamoto Tetsuya . Matsuda Tadashi . Sep 2003 . Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3 . J. Biol. Chem. . 278 . 36 . 34253–8 . 0021-9258. 12815042 . 10.1074/jbc.M304961200 . free . 2115/28123 . free .
4. Sachdev . S . Bruhn L . Sieber H . Pichler A . Melchior F . Grosschedl R . Dec 2001 . PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies . Genes Dev. . 15 . 23 . 3088–103 . 0890-9369. 11731474 . 10.1101/gad.944801 . 312834 .
5. July 2024 . Rodríguez Pérez F, Natwick D, Schiff L, McSwiggen D, Heckert A, Huey M, Morrison H, Loo M, Miranda RG, Filbin J, Ortega J, Van Buren K, Murnock D, Tao A, Butler R, Cheng K, Tarvestad W, Zhang Z, Gonzalez E, Miller RM, Kelly M, Tang Y, Ho J, Anderson D, Bashore C, Basham S . WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis . Nat. Commun. . 15 . 6059 . 6059 . 39025847. 11258360 . 10.1038/s41467-024-50178-3. 2024NatCo..15.6059R . free.